Quantigene plex assay user manual

• QuantiGene™ Plex Assay Kit: contains the generic reagents, plates, and seals required for running the assay • QuantiGene™ Plex Panel: contains the custom target-specific pooled probe set and associated magnetic capture beads to capture user-defined genes of interest QuantiGene™ Plex Gene Expression Assay User Guide 5. Apr 01,  · Detailed protocols for ProcartaPlex and QuantiGene Plex Assays. Finally, 40 μL of homogenate of each sample were tested in triplicate using the plex gene panel. Samples USA) and data analysis was performed using Bio-plex Manager software. The multiplex bDNA assays were run as recommended in the QuantiGene ™ Plex Reagent System User Manual. QuantiGene ® ViewRNA ISH Cell Assay User Manual. We tested the branched-chain DNA (bDNA) technology QuantiGene Reagent System for quantifying the expression of RNA in formalin-fixed tissues.

Invitrogen HepaRG™ Cell User Guide, version from 2/13/ Affymetrix QuantiGene Plex Assay User Manual, P/N Rev. Probe design software was used to generate probe sets for singleplex or multiplex bDNA assays. Plex MAGPIX system, refer to the assay instruction manual for correct bead regions and bead type.

This manual provides complete instructions for performing QuantiGene ViewRNA assays for adherent cells in either. Both the Blue Module and the Core Kit are required to perform a ViewRNA ISH Tissue 2-plex assay. QP).

The Rat Kidney Tissue Slides., call About This User Manual Who This Manual is For This manual is for anyone who has purchased QuantiGene Plex DNA Assay Kit, DNA Plex Set and intends to utilize a magnetic plate washer to perform the QuantiGene Plex DNA assay for any of the following sample types: Cultured cells Fresh, frozen, or formalin-fixed, paraffin-embedded (FFPE) tissues. Date RNAscope® 2-Plex Detection Kit (Chromogenic) User Manual PART 2 Catalog Number For. As demonstrated in Figure 7, the fluorescent signal of an RNA species is consistent across the entire plate.

quantigene plex assay user manual Meet Ankit Patel, QuantiGene™ Assay Developer at Thermo Fisher Scientific March 26th, / Hilary Graham.QuantiGene® ViewRNA probe sets are ordered separately. quantigene plex assay user manual The QuantiGene™ Plex DNA Assay Kit is designed for use with QuantiGene ™ DNA Plex Sets and QuantiGene Sample Processing Kits (not required for purified DNA samples) for determination of copy number variation directly from a variety of sample types. Kit size Component [1,2] Quantity Storage QS 2 plates Proteinase K (50 µg/µL) Lysis Mixture µL 10 mL –20°C 15–30°C. No. Plus: 6 tips for QuantiGene assays When you want an assay that can detect nucleic acids, you’ll likely choose the high-quality Invitrogen™ ProcartaPlex™ and QuantiGene™ Plex assays from Thermo Fisher Scientific, a Luminex partner.

Kit size Component [1,2] Quantity Storage QS 2 plates Proteinase K (50 µg/µL) Lysis Mixture µL 10 mL –20°C 15–30°C. For quantitating RNA targets, we recommend the use of QuantiGene Sample Assessment Kit to evaluate relative cell number and RNA quality of tissue homogenates. The multiplex bDNA assays were run as recommended in the QuantiGene™ Plex Reagent System User Manual. QuantiGene Plex Gene Expression Assay Accurate and precise RNA quantitation—measure up to 80 genes in a single well QuantiGene Plex assays provide a fast and high-throughput solution quantigene plex assay user manual for multiplexed gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a or well plate. Overview of the QuantiGene Plex System. 12,13,14,15 Cited by: • QuantiGene™ Plex Assay Kit: contains the generic reagents, plates, and seals required for running quantigene plex assay user manual the assay • QuantiGene™ Plex Panel: contains the custom target-specific pooled quantigene plex assay user manual probe set and associated magnetic capture beads to capture user-defined genes of interest QuantiGene™ Plex Gene Expression Assay User Guide 5.

Cook, Brian C. U-PLEX Biomarker Group 1 (ms) Description - This Group is an extensive combination of assays for non-human primate cytokines and chemokines that are involved in many biological processes such as inflammation, the Th1, Th2, and Th17 quantigene plex assay user manual pathways, chemotaxis, angiogenesis, and immune system regulation. Refer to the QuantiGene Singleplex User Manual and QuantiGene Plex Assay User Manual for more information about running the assays. Apr 01, · Measurement of mRNA expression levels with QuantiGene Human plex Panel.

Verbist, Eva Adriaensen, Vikki Keersmaekers, Dea Putri, Marjolein Crabbe, Maarten Derks, R. This technical note should be used in conjunction with the QuantiGene ViewRNA ISH Tissue Assay User Manual and the Little. No. Preparation of bacterial homogenates for use in QuantiGene and QuantiGene Plex Assays; Factors affecting blood gene expression; Defining a QuantiGene Plex panel; User manuals User manuals for use with assay kits QP, QP, and QP QGP manual for assays with plex and plex; QuantiGene sample processing kit product inserts. The Bio-Plex validation kit consists of beads to evaluate the following components of the Bio-Plex suspension array system: 1) optics alignment, quantigene plex assay user manual 2) integrity of fluidics, 3) reporter channel performance, and 4) .

QuantiGene Plex Assays for RNA targets and QuantiGene Plex DNA assays for DNA targets. Verbist, Eva Adriaensen, Vikki Keersmaekers, Dea Putri, Marjolein Crabbe, Maarten Derks, R. Bio-Plex Pro™ Cytokine, Chemokine, and Growth Factor Assays Instruction Manual For technical support, call your local Bio-Rad office, or in the U. Cooperative hybridization and subsequent quantification were performed following the manufacturer’s recommended procedure for frozen animal tissue (see Quantigene® Plex Assay User Manual, Affymetrix). Expected Results. Probe sets were designed and synthesized Cited by: 7. Cat. For the multiplex bead immunoassay the Human Inflammatory 5-plex panel according to Invitrogen's user manual 'Human Inflammatory 5-Plex Panel'.

Cooperative quantigene plex assay user manual hybridization and subsequent quantification were performed following the manufacturer’s recommended procedure for frozen animal tissue (see Quantigene® Plex Assay User Manual, Affymetrix). 8 This sandwich nucleic acid hybridization assay provides a unique advantage over existing methods by amplifying the reporter signal and by avoiding enzymatic amplification of the target RNA. The bDNA assay is a hybridization-based method quantigene plex assay user manual of target-specific RNA quantitation. from five independent QuantiGene® runs (Table 1). Life Science Instruments Reagents PCR and RT-PCR Cloning Real-Time PCR Cell Biology Flow cytometry Obesity Research Stem Cells Immunohistochemistry Cytogenesis Protein Function Related products ProcartaPlex Multiplex immunoassays Quantigene Plex Diagnostics Instruments Reagents Laboratory products Plastic and Consumables Safety Data Sheets. The QuantiGene ® Plex assay kit from Affymetrix offers a means to quantitate multiple mRNA species simultaneously from a variety of different sample types. The mRNA specific Probe Sets (heated to 95º for 5 minutes immediately prior to use), Lysis Mixture, mRNA specific Capture Beads, Proteinase K and Blocking Reagent were combined according to the kit instructions to make Working Plex set.

The U-PLEX platform allows you to create custom panels quickly in your own lab from a selection of MSD assays, your own antibodies, or a combination of both. Refer to the QuantiGene Singleplex User Manual quantigene plex assay user manual and QuantiGene Plex Assay User Manual for more information about running the assays. quantigene plex assay user manual Contents and storage Refer to product label for expiration date. Proportional volumes of reagents/well were used for the ‐well [HOST] by: USER MANUAL For Molecular Biology Applications, not intended for diagnosis.

• QuantiGene™ Singleplex Assay Kit: contains the generic reagents, plates, and seals required for running the assay. Simple Western technical library. Refer to the appropriate QuantiGene Vi ewRNA ISH Tissue Assay User Manual, based on equipment used, for instructions on performing in situ hybridization assays. The mRNA specific Probe Sets (heated to 95º for 5 minutes immediately prior to use), Lysis Mixture, mRNA specific Capture Beads, Proteinase K and Blocking Reagent were combined according to the kit instructions to make Working Plex set.

All reagents described below were purchased from Affymetrix unless [HOST] by: 4. The QGP. Cell lysates were prepared according to manufacturer’s in-structions, using the QuantiGene® Sample Processing Kit (Cultured Cells), while the QuantiGene® assay was run on a Luminex FlexMAP® 3D platform, following the QuantiGene® Plex user manual, distributed by Ther-moFisher Author: Bie M.. ViewRNA ISH Tissue Assay, Blue Module (for 2-plex assays only): contains reagents for the detection of a second RNA target using the Fast Blue Substrate. This user guide contains instructions for using the QuantiGene™ Plex Assay with the.

Preparation of quantigene plex assay user manual bacterial homogenates for use in QuantiGene and QuantiGene Plex Assays; Factors affecting blood gene expression; Defining a QuantiGene Plex panel; User manuals User manuals for use with assay kits QP, QP, and QP QGP manual for assays with plex and plex; QuantiGene sample processing kit product inserts.C 2/9/ Cryopreserved HepaRG™ Cells: An Alternative In Vitro Screening Tool for Human Hepatic Drug Metabolism, Induction of Metabolism, & Safety Applications, Jonathan P. Contents and Storage The QuantiGene ViewRNA ISH Tissue Positi ve Control Kit contains the following components. Species mammalian Plex level Up to 3 RNA targets Assay format mL microfuge tube Detection fluorescent Instrumentation for detection Standard flow cytometer equipped with blue ( nm) and red ( nm or similar) lasers, and filter sets for FITC, APC and APC-Cy7 2 QuantiGene® FlowRNA Assay User Manual How it Works The QuantiGene® FlowRNA.

The assay described in this manual is designed for cells grown on glass cover slips in well plate(s) following the assay specifications provided in Table on page 2. Cell lysates were prepared according to manufacturer’s in-structions, using the QuantiGene® Sample Processing Kit (Cultured Cells), while the QuantiGene® assay was run on a Luminex FlexMAP® 3D platform, following the QuantiGene® Plex user manual, distributed by Ther-moFisher. Rev.

Overview of quantigene plex assay user manual the QuantiGene Plex Assay The QuantiGene Plex (QGP) assay is the most accurate and precise assay for multiplexed gene expression quan-titation. Aug 01, · Quantigene plex assay procedure. Figure. For the quantification of RNA quantigene plex assay user manual in archival material, the Invitrogen™ QuantiGene™ Plex Assay is a fast and efficient multiplex solution using branched DNA (bDNA) technology on . QuantiGene Plex ‐Well Assay Protocol. Using the Luminex ® xMAP technology, QGP assays allow the simultaneous measurement of 3 .

PURPOSE: This protocol describes the procedure for performing, single-plex QuantiGene assays with purified RNA using the LMAX luminometer. ProcartaPlex and QuantiGene Plex assays measure secreted proteins and mRNA expression Beads were allowed to settle for 2 min and then the liquid was decanted with a manual pipette. About This Manual The QuantiGene ViewRNA HC Screening assay is an in situ hybridization method for visualization of up to 4 target RNAs, at single transcript detection level, simultaneously within individual cells. Catalog Number. Materials and Methods.

Bio-Plex Pro™ Cytokine, Chemokine, and Growth Factor Assays Instruction Manual For technical support, call your local Bio-Rad office, or in the U. All genes were normalized to GAPDH and data is provided on the attached Excel files. The bDNA assay is a hybridization-based method of target-specific RNA quantitation.

For more information, refer to the appropriate user manual. • QuantiGene™ Singleplex Assay Kit: contains the generic reagents, plates, and seals required for running the assay. GAPDH is used as a housekeeping quantigene plex assay user manual gene. Cell lysates were prepared according to manufacturer’s instructions, using the QuantiGene® Sample Processing Kit (Cultured Cells), while the QuantiGene® assay was run on a Luminex FlexMAP® 3D platform, following the QuantiGene® Plex user manual, distributed quantigene plex assay user manual by ThermoFisher [HOST]: Bie M. quantigene plex assay user manual QuantiGene Plex Gene Expression Assay Accurate and precise RNA quantitation—measure up to 80 genes in a single well QuantiGene Plex assays provide a fast and high-throughput solution for multiplexed gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a or well plate.

For more information about the components provided in the assay, please refer to the User Manual, QuantiGene® ViewRNA for FFPE Samples, available at [HOST] Figure 2: Workflow of the in situ hybridization assay based on bDNA technology for (A) cultured cells and (B) FFPE. • QuantiGene™ quantigene plex assay user manual Singleplex Probe Set: contains the custom target-specific pooled probe set to capture user-defined gene of interest. For th ese control FFPE slides, use a 10 minute pretreatment boiling time and a 20 minute Protease incubation time as described in the QuantiGene ViewRNA ISH Tissue Assay User Manual. Contents and storage Refer to product label for expiration date. Spp1 and TYPE quantigene plex assay user manual 6 rat Ubc Probe Se ts. quantigene plex assay user manual Please note that the general Technical Library page may contain additional product categories of interest. Jackson et al.

This user guide contains instructions for using the QuantiGene™ Plex Assay with the. Sample Preparation Pretreatment Guide for Formalin-Fixed Paraffin-Embedded (FFPE), see. This manual provides complete instructions for performing QuantiGene ViewRNA assays for adherent cells in either: An automated format for or well plates or. Aug 01,  · Quantigene plex assay procedure. 8 This sandwich nucleic acid hybridization assay provides a unique advantage over existing methods by amplifying the reporter signal and by avoiding enzymatic amplification of the target RNA.

Protocols for the following formats are listed in the appendices. Overview of the QuantiGene Plex Assay The QuantiGene Plex (QGP) assay is the most accurate and precise assay for multiplexed gene expression quan-titation. QuantiGene Assay SOP. Part 1. The QuantiGene Plex ‐well Assay was optimized using technical support from Affymetrix and using the 96‐well assay protocol provided by the manufacturer.

This technical note should be used in conjunction with the QuantiGene ViewRNA ISH Tissue Assay User Manual and the quantigene plex assay user manual Little. For research use only. GAPDH is used as a housekeeping gene., call All the assays were run according to the QuantiGene Plex User Manual using Panel A total of QuantiGene Plex Assay plates were processed.

Cell lysates were prepared according to manufacturer’s instructions, using the QuantiGene® Sample Processing Kit (Cultured Cells), while the QuantiGene® assay was run on a Luminex FlexMAP® 3D platform, following the QuantiGene® Plex user manual, distributed by ThermoFisher Scientific. Step 1. Brotski, Shelley Das, Markus Miholits. For more information on this product, please refer to the QuantiGene™ Plex DNA Assay User Manual. The bDNA assay is a hybridization-based method of target-specific RNA quantitation.B User Manual quantigene plex assay user manual QuantiGene® ViewRNA ISH Tissue 2-Plex Assay Equipment: ThermoBrite Hybridization System ThermoBrite® This programmable system automates the denaturation and hybridization steps in slide-based (F)ISH procedures, and provides walk-away convenience for. • QuantiGene™ Singleplex Probe Set: contains the quantigene plex assay user manual custom target-specific pooled probe set to capture user-defined gene of interest. QuantiGene® Plex Assays were performed as described in the assay kit instructions.

PURPOSE: This protocol describes the procedure for performing, single-plex QuantiGene assays with purified RNA using the LMAX luminometer. Customize a U-PLEX Assay With the Assay Designer. QuantiGene Assay SOP. Using the Luminex ® xMAP technology, QGP quantigene plex assay user manual assays allow the simultaneous measurement of 3 to 80 mRNA species in every well of a well plate. Related User Documents Refer to the QuantiGene ViewRNA miRNA ISH Cell Assay User Manual, format: well Optical-Botttom Plate for assay instructions using cultured cell samples processed in a well optical-bottom plate.

We tested the branched-chain DNA (bDNA) technology QuantiGene Reagent System quantigene plex assay user manual for quantifying the expression of RNA in formalin-fixed tissues. For more information on this product, please refer to the QuantiGene™ Plex DNA Assay User Manual. Probe design software [22] was used to generate probe sets for singleplex or multiplex.

Samples USA) and data analysis was performed using Bio-plex Manager software. and quality control tested for the end user. QuantiGene ® ViewRNA ISH Cell Assay User Manual. P.

Disease Focus/Application Area - Wide ranging, including multiple sclerosis, Crohn’s . Montoya, Chris M. The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument. Then 6, and 12, cells were seeded per well of a ‐well plate. The assay described in this manual is designed for cells grown on glass cover slips in well plate(s) following the assay specifications provided in Table on page 2.

Preparation of bacterial homogenates for use in QuantiGene SinglePlex and QuantiGene Plex assays Recent publications You may use any term to search the publications list by entering a search term and clicking Search. Design your customized U-PLEX panel by following the steps below. Refer to the QuantiGene ViewRNA miRNA ISH Cell Assay Supplemental Reference Guide for the following information. For more information about the components provided in the assay, please refer to the User Manual, QuantiGene® ViewRNA for FFPE Samples, available at [HOST] quantigene plex assay user manual Figure 2: Workflow of the in situ hybridization assay based on bDNA technology for (A) cultured cells and (B) FFPE.

About This Manual The QuantiGene ViewRNA HC Screening assay is an in situ hybridization method for visualization of up to 4 target RNAs, at single transcript . The QuantiGene Plex assay combines branched DNA (bDNA) signal amplification and multi-analyte profiling beads (xMAP ®) technologies to enable the detection and quantitation of multiple mRNA targets simultaneously. P/N Rev. Cat. Species mammalian Plex level Up to 3 RNA targets Assay format mL microfuge tube Detection fluorescent Instrumentation for detection Standard flow cytometer equipped with blue ( nm) and red ( nm or similar) lasers, and filter sets for FITC, APC and APC-Cy7 2 QuantiGene® FlowRNA Assay User Manual How it Works The QuantiGene® FlowRNA.

12,13,14, The QuantiGene Plex assay combines branched DNA (bDNA) signal amplification and multi-analyte profiling beads (xMAP ®) technologies to enable the detection and quantitation of multiple mRNA targets simultaneously. QuantiGene® Plex Assays were performed as described in the assay kit instructions. This technology, which uses ferrite beads as a solid substrate for the reactions requires a magnetic field . µL of wash buffer was added to each well Author: Damon B., Life Technologies, poster. No. For research use only.

The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets. The assay is designed to be compatible in batch or completely automated modes using BioTek’s ELx Magnetic Bead Washer.S. 2 QuantiGene ® miRNA Assay User Manual QuantiGene miRNA Assay Basics The QuantiGene miRNA Assay enables researchers to perform direct detection and quantification of miRNAs at single-base resolution, avoiding biases associated with.

Nov 06, · By using a plex assay of house-keeping genes in conjunction with purified known RNA samples, the uniformity of signal response when using the ELx Magnetic Bead quantigene plex assay user manual Washer to wash the assay in well microplates can be examined. QuantiGene® ViewRNA probe sets are ordered separately. The bDNA assay is a hybridization-based method of target-specific RNA quantitation. Protocols for the following formats are listed in the appendices. The QuantiGene™ Plex DNA Assay Kit is designed for use with QuantiGene ™ DNA Plex Sets and QuantiGene Sample Processing Kits (not required for purified DNA samples) for determination of copy number variation directly quantigene plex assay user manual from a variety of sample types. All genes were normalized to GAPDH and data is provided on the attached Excel files. Probe design software was used to generate probe sets for singleplex or multiplex bDNA assays.

Our study demonstrates for the first time that a branched DNA Cited by: 5. The multiplex bDNA assays were run as recommended in quantigene plex assay user manual the QuantiGene™ Plex Reagent System User Manual. For the quantification of RNA in archival material, the Invitrogen™ QuantiGene™ Plex Assay is a fast and efficient multiplex solution using branched DNA (bDNA) technology on Luminex technology–based magnetic beads. 2 QuantiGene® Plex Assay User Manual How it Works The QuantiGene Plex assay combines branched DNA (bDNA) signal amplification quantigene plex assay user manual and multi-analyte quantigene plex assay user manual profiling beads (xMAP®) technologies to enable the detection and quantitation of multiple RNA targets simultaneously. QuantiGene® Plex Assays were performed as described in the assay kit instructions. The QuantiGene ViewRNA Chemiluminescent Assay is an in situ hybridization method for quantification of target RNA, in the cytoplasms of fixed cells. Figure 2. Finally, 40 μL of homogenate of each sample were tested in triplicate using the plex gene panel.

Probe sets were designed and synthesized. About This Manual The QuantiGene ViewRNA Chemiluminescent Assay is an in situ hybridization method for quantification of target RNA, in the cytoplasms of fixed cells. An identical biomarker configuration for mRNA using a QuantiGene plex Panel (Thermo Fisher Scientific, custom) and the Invitrogen™ QuantiGene™ Plex Assay Kit for the generic reagents (Thermo Fisher Scientific quantigene plex assay user manual Cat. QuantiGene Plex DNA CNV Assay Literature & Support QuantiGene Plex Assays for DNA Copy Number; User manual QuantiGene Plex DNA Assay.

2 QuantiGene® Plex Assay User Manual How it Works The QuantiGene Plex assay combines branched DNA (bDNA) signal amplification and multi-analyte profiling quantigene plex assay user manual beads (xMAP®) technologies to enable the detection quantigene plex assay user manual and quantitation of multiple RNA targets simultaneously. McLucas, Leticia A. For the multiplex bead immunoassay the Human Inflammatory 5-plex panel according to Invitrogen's user manual 'Human Inflammatory 5-Plex Panel'. For more information, see the QuantiGene. All reagents described below were purchased from Affymetrix unless noted. user to discriminate between assay and instrumentation problems.

P. negative (omit probe sets) control assay using a total of 2 ra t kidney tissue sections. For a list of peer-reviewed articles our products have been cited in, check out the publications page. Guidelines and procedure are provided for running the quantigene plex assay user manual QuantiGene ViewRNA ISH Tissue Assay using the Little Dipper Processor for Affymetrix from SciGene for batch slide processing and the ThermoBrite from Abbott Molecular. Plex MAGPIX system, refer to the assay instruction manual for correct bead regions quantigene plex assay user manual and bead type. Bio-Plex® System Hardware Instruction Manual Catalog Numbers , , , For technical support, call your local Bio-Rad office or, in the US, call BIORAD ().

Jul 01,  · We developed and evaluated a multiplex branched DNA assay for the detection and subtyping of avian influenza (AI) virus strains. Guidelines and procedure are provided for running the QuantiGene ViewRNA ISH Tissue Assay using the Little Dipper Processor for Affymetrix from SciGene for batch slide processing and the ThermoBrite from Abbott Molecular. The assay successfully detected all 94 AI virus strains of 15 different hemagglutinin (HA) subtypes tested quantigene plex assay user manual while simultaneously differentiating 24 North American H5, 11 Eurasian H5, and 11 H7 strains.

ViewRNA TYPE 1 and TYPE 6 Probe Sets: contains oligonucleotides. from five independent QuantiGene® runs (Table 1). All the assays were run according to the QuantiGene Plex User Manual using Panel A total of QuantiGene Plex Assay plates were processed.S.


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